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- Title
Forensic application of EST-derived STR markers in opium poppy.
- Authors
MIČIANOVÁ, Veronika; ONDREIČKOVÁ, Katarína; MUCHOVÁ, Darina; KLČOVÁ, Lenka; HUDCOVICOVÁ, Martina; HAVRLENTOVÁ, Michaela; MIHÁLIK, Daniel; KRAIC, Ján
- Abstract
Discrimination study in opium poppy (Papaver somniferum L.) genotypes has been performed by short tandem repeat (STR) markers derived from expressed sequence tags (ESTs) retrieved from the opium poppy DNA. Some of STRs originate from the gene sequences involved in the biosynthesis of morphine. Eight of twelve tested EST-derived STR markers generated polymorphism, 2-4 alleles were identified per locus, and the mean polymorphic information content value was 0.338. Seven cultivars developed in Slovakia, registered and released at present time for commercial cultivation were discriminated mutually. All the Slovak and Czech cultivars were also different from each other. EST-derived STR markers have shown a potential for discrimination of opium poppy genotypes cultivated for commercial production of either edible seeds or pharmacologically active alkaloids and could be applicable for the PCR-based forensic analyses.
- Subjects
OPIUM poppy; SHORT tandem repeat analysis; EXPRESSED sequence tag (Genetics); GENE expression in plants; NUCLEOTIDE sequence
- Publication
Biologia, 2017, Vol 72, Issue 6, p587
- ISSN
0006-3088
- Publication type
Article
- DOI
10.1515/biolog-2017-0076