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- Title
New Insight into the Concanavalin A-Induced Apoptosis in Hepatocyte of an Animal Model: Possible Involvement of Caspase-Independent Pathway.
- Authors
Zhao, Xiangli; Fu, Cheng; Sun, Lingjuan; Feng, Hao; Xie, Peiling; Wu, Meng; Tan, Xiaosheng; Chen, Gang
- Abstract
Concanavalin A (Con A) is known to be a T-cell mitogen and has been shown to induce hepatitis in mice through the triggering of conventional T cells and NKT cells. However, it remains unknown whether Con A itself can directly induce rapid hepatocyte death in the absence of a functional immune system. Here, by using an immunodeficient mouse model, we found Con A rapidly induced liver injury in vivo despite a lack of immunocyte involvement. We further observed in vitro that hepatocytes underwent a dose-dependent but caspase-independent apoptosis in response to Con A stimulation in vitro. Moreover, transcriptome RNA-sequencing analysis revealed that apoptosis pathways were activated in both our in vivo and in vitro models. We conclude that Con A can directly induce rapid but non-classical apoptosis in hepatocytes without the participation of immunocytes. These findings provide new insights into the mechanism of Con A-induced hepatitis.
- Subjects
APOPTOSIS; ANIMAL models in research; LIVER cells; T cells; CONCANAVALIN A; LIVER injuries
- Publication
Molecules, 2023, Vol 28, Issue 3, p1312
- ISSN
1420-3049
- Publication type
Article
- DOI
10.3390/molecules28031312