We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Detection and localization of two hydrogenases in Methylococcus capsulatus (Bath) and their potential role in methane metabolism.
- Authors
Hanczár, Tímea; Csáki, Robert; Bodrossy, Levente; Murrell, Colin J.; Kovács, Kornél L.
- Abstract
Methylococcus capsulatus (Bath) was shown to contain two distinct hydrogenases, a soluble hydrogenase and a membrane-bound hydrogenase. This is the first report of a membrane-bound hydrogenase in methanotrophs. Both enzymes were expressed apparently constitutively under normal growth conditions. The soluble hydrogenase was capable of reducing NAD+ with molecular hydrogen. The activities of both soluble and particulate methane monooxygenases could be driven by molecular hydrogen. This confirmed that molecular hydrogen could be used as a source of reducing power for methane oxidation. Hydrogen-driven methane monooxygenase activities tolerated elevated temperatures and moderate oxygen concentrations. The significance of these findings for biotechnological applications of methanotrophs is discussed.
- Subjects
HYDROGENASE; METALLOENZYMES; OXYGENASES; BACTERIA; NAD (Coenzyme); HYDROGEN; METHANE
- Publication
Archives of Microbiology, 2002, Vol 177, Issue 2, p167
- ISSN
0302-8933
- Publication type
Article
- DOI
10.1007/s00203-001-0372-4