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- Title
Molecular characterization of the Sinorhizobium meliloti nlpD gene.
- Authors
Streit, Wolfgang R.; Hofmann, Kai; Liebl, Wolfgang
- Abstract
The Sinorhizobium meliloti nlpD gene consists of 1,539 nucleotides and codes for 512 amino acids. Expression of the nlpD gene as a histidine-tagged protein in Escherichia coli resulted in the production of a 57-kDa protein. The deduced polypeptide sequence of NlpD contains one unusual hexamer repeat (KVQRGQ), one tetramer (TVTV) and two direct and inverted trimer repeats (KAA, AAK). The N-terminal amino acid residues displayed similarity with signal peptides of secreted bacterial lipoproteins. Mutations of the S. meliloti nlpD gene caused decreased survival of cells in the stationary phase.
- Subjects
ESCHERICHIA coli; PEPTIDES; PROKARYOTES; ENTEROBACTERIACEAE; PSEUDOMONAS aeruginosa; MICROBIOLOGY
- Publication
Archives of Microbiology, 2000, Vol 174, Issue 4, p292
- ISSN
0302-8933
- Publication type
Article
- DOI
10.1007/s002030000205