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- Title
Sutureless Surgical Orthotopic Implantation Technique of Primary and Metastatic Cancer in the Liver of Mouse Models.
- Authors
HIROTO NISHINO; HOLLANDSWORTH, HANNAH M.; NORIHIKO SUGISAWA; JUN YAMAMOTO; YOSHIHIKO TASHIRO; SACHIKO INUBUSHI; KAZUYUKI HAMADA; YU SUN; HYEIN LIM; AMIRFAKHRI, SIAMAK; FILEMONI, FILEMONI; HOFFMAN, ROBERT M.; BOUVET, MICHAEL
- Abstract
Background/Aim: Surgical orthotopic implantation (SOI) is used to establish patient-derived orthotopic xenograft (PDOX) and other orthotopic mouse models. Orthotopic liver models can be challenging, as the liver parenchyma is prone to bleeding. The present report describes a sutureless method to implant tumors in the liver that reduces bleeding and procedural time. Materials and Methods: Human HCC cell-line (Huh-7-GFP) and CM2, a patient-derived coloncancer liver metastasis, were used for sutureless SOI of tumor fragments in the liver of nude mice. A small cavity was formed on the liver surface. A solitary tumor fragment was implanted in the cavity without suturing to create hemostasis. Results: Six weeks after sutureless SOI, the tumor volume of Huh-7-GFP (n=5) was 584.41±147.64 mm3 and the tumor volume of CM2 (n=5) was 1336.54±1038.20 mm3. The engraftment rate was 100%. Conclusion: This novel method for establishing orthotopic liver-implantation mouse models is suitable for studies of liver cancer and liver metastases due to its simple procedure and potential high engraftment rate.
- Subjects
XENOTRANSPLANTATION; LIVER cancer; LIVER metastasis; COLON cancer; CANCER invasiveness; ANIMAL models of cancer; ONCOLOGIC surgery
- Publication
In Vivo, 2020, Vol 34, Issue 6, p3153
- ISSN
0258-851X
- Publication type
Article
- DOI
10.21873/invivo.12149