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- Title
Effect of Taurine on Turkey (Meleagris gallopavo) Spermatozoa Viability and Motility.
- Authors
SLANINA, TOMÁŠ; MIŠKEJE, MICHAL; TIRPÁK, FILIP; BŁASZCZYK, MARTYNA; STAWARZ, ROBERT; MASSÁNYI, PETER
- Abstract
The effect of taurine on the turkey spermatozoa motility and viability during the in vitro incubation was assessed. Experimental samples were prepared by diluting the raw semen in nine different concentrations of taurine - from 10 mg/ml to 0.078125 mg/ml. The motility parameters were evaluated by the CASA system (Computer Assisted Semen Analyser) using the program Sperm Vision® and for spermatozoa viability assessment the eosin-nigrosin staining was performed. Selected parameters were evaluated at six time periods: 0, 1, 2, 3, 4, and 5 h at 5°C and 41°C. At 5°C, a significantly lower percentage of motility and progressive motility was detected only in the samples with the highest concentration of taurine (10 mg/ml) at time 0 and 1. After 2 h of incubation a significant preventive effect of taurine on spermatozoa parameters was observed. The tendency of the taurine effect on motility parameters was different during the in vitro incubation at 41°C. Significantly lower values of motility parameters were detected in all experimental samples in comparison to the control after 5 h. The analysed concentrations of taurine did not significantly affect viability of turkey spermatozoa during all time periods. A higher percentage of dead spermatozoa were observed at 41°C (4.87-9.90%) if compared to 5°C (2.12-4.88%). The results indicated that the addition of taurine (from 2.5 to 7.5 mg/ml) to turkey spermatozoa positively affected the monitored spermatozoa parameters incubated at 5°C.
- Subjects
TURKEY embryology; TAURINE; SPERM motility; CELL survival; CELL membranes
- Publication
Czech Journal of Animal Science, 2018, Vol 63, Issue 4, p127
- ISSN
1212-1819
- Publication type
Article
- DOI
10.17221/79/2017-CJAS