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- Title
Effects of the Fas/Fas-L pathway on fluoride-induced apoptosis in SH-SY5Y cells.
- Authors
Bayi Xu; Zhixia Xu; Tao Xia; Ping He; Ping Gao; Weihong He; Ming Zhang; Lijuan Guo; Qiang Niu; Aiguo Wang
- Abstract
The mechanisms underlying fluoride-induced apoptosis in neurons still remain unknown. To investigate apoptosis, caspase-3 activity, and mRNA expression of Fas, Fas-L, and caspases (-3 and -8) induced by fluoride, human neuroblastoma (SH-SY5Y) cells were incubated with 0, 20, 40, and 80 mg/L sodium fluoride (NaF) for 24 h in vitro. The data show that cell viability in the 40 and 80 mg/L fluoride groups were significantly lower than that of the control group. The percentages of apoptosis in the 40 and 80 mg/L fluoride groups were markedly higher than those in the control group, and they increased with the increase in fluoride concentration. The activity of caspase-3 and mRNA expression levels for Fas, Fas-L, and caspases (-3 and -8) in the 40 and 80 mg/L fluoride groups were significantly higher than those in the control group. An agonistic anti-Fas monoclonal antibody (CH-11) significantly augmented apoptosis induction by fluoride, showing a synergistic effect, while a Fas-blocking antibody (ZB4) partly inhibited fluoride-induced apoptosis of SH-SY5Y cells. The results indicate that fluoride exposure could induce apoptosis in SH-SY5Y cells, and the Fas/Fas-L signaling pathway may play an important role in the process. © 2009 Wiley Periodicals, Inc. Environ Toxicol 26: 86-92, 2011.
- Subjects
APOPTOSIS; PHYSIOLOGICAL effects of fluorides; NEURONS; TOXICOLOGY of fluorine; MESSENGER RNA; NEUROBLASTOMA; MONOCLONAL antibodies; NEURODEGENERATION; FLOW cytometry
- Publication
Environmental Toxicology, 2011, Vol 26, Issue 1, p86
- ISSN
1520-4081
- Publication type
Article
- DOI
10.1002/tox.20543