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- Title
Human Liver Catalase: Cloning, Expression and Characterization of Monoclonal Antibodies.
- Authors
Jin, Li Hua; Kim, Dae Won; Eum, Won Sik; Yoon, Chang Sik; Jang, Sang Ho; Choi, Hee Soon; Choi, Soo Hyun; Kim, Young Hoon; Kim, So Young; Jung, Mi Ryoung; Kang, Tae-Cheon; Ho, Moo; Hyeon, Won; Lee, Yong; Kang, Jung Hoon; Kwon, Oh-Shin; Cho, Sung-Woo; Lee, Kil Soo; Park, Jinseu; Choi, Soo Young
- Abstract
We isolated a cDNA encoding liver catalase from a human liver eDNA library. The eDNA had a high degree of sequence similarity to the corresponding enzyme from other sources. It was expressed in E. coil using the pET15b vector. The protein produced was enzymatically active after purification, and its kinetic parameters closely resembled those of other mammalian catalases. Monoclonal antibodies were generated against the purified catalase; six antibodies recognizing different epitopes were obtained, one of which inhibited the enzyme. The cross reactions of the antibodies with brain catalases from human and other mammalian tissues were investigated, and all the immunoreactive bands obtained on Western blots had molecular masses of about 58 kDa, Similarly fractionated extracts of several mammalian cell lines all gave a single band or" molecular mass 58 kDa. These results indicate that mammalian livers and human cell lines contain only one major type of immunologically reactive catalase, even though some of catalases have been previously reported to differ in certain properties.
- Subjects
MONOCLONAL antibodies; DNA; LIVER; ESCHERICHIA coli; BACTERIA
- Publication
Molecules & Cells (Springer Nature), 2003, Vol 15, Issue 3, p381
- ISSN
1016-8478
- Publication type
Article
- DOI
10.1016/s1016-8478(23)13754-x