We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Construction of conditionally replicating adenovirus expressing staphylococcal enterotoxin A gene: potential usefulness for anti-tumor therapies.
- Authors
ZHANG, P.-Y.; HAO, L.; ZHANG, Z.-G.; DONG, B.-Z.; YANG, D.; WANG, X.-L.; XUAN, X.-J.; YAN, Z.; QING, L.; SHI, Z.-D.; LIU, D.; HAN, C.-H.
- Abstract
OBJECTIVE: The aim of this study was to construct a conditionally replicating adenovirus pPE3-SEA expressing staphylococcal enterotoxin A (SEA) gene. MATERIALS AND METHODS: A full-length SEA gene fragment was cloned into pENTR12 plasmid to obtain a recombinant viral plasmid pENTR12-SEA. The pENTR12-SEA plasmid was co-transfected into HEK293 cells along with pPE3-ccdB, which encoded for the virus backbone, to generate recombinant adenovirus pPE3-SEA vector. Amplified pPE3-SEA vectors were purified, and viral titer was determined using the 50% tissue culture infective dose method. RESULTS: The PCR, restriction enzyme digestion, and sequence analyses proved successful construction of replicating oncolytic adenovirus pENTR12-SEA and recombinant SEA expressing oncolytic adenovirus pPE3-SEA. The viral titer was 2.5 x 1010 pfu/ml. CONCLUSIONS: We successfully constructed conditionally replicating adenovirus pPE3-SEA which can be utilized for experimental studies of tumor-targeted therapies.
- Subjects
ADENOVIRUSES; ENTEROTOXINS; TUMOR treatment; GENES; CHROMOSOME replication; THERAPEUTICS
- Publication
European Review for Medical & Pharmacological Sciences, 2014, Vol 18, Issue 16, p2258
- ISSN
1128-3602
- Publication type
Article