We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Exploring the Glucose Fluxotype of the E. coli y-ome Using High-Resolution Fluxomics.
- Authors
Bergès, Cécilia; Cahoreau, Edern; Millard, Pierre; Enjalbert, Brice; Dinclaux, Mickael; Heuillet, Maud; Kulyk, Hanna; Gales, Lara; Butin, Noémie; Chazalviel, Maxime; Palama, Tony; Guionnet, Matthieu; Sokol, Sergueï; Peyriga, Lindsay; Bellvert, Floriant; Heux, Stéphanie; Portais, Jean-Charles; Villas-Boas, Silas G.
- Abstract
We have developed a robust workflow to measure high-resolution fluxotypes (metabolic flux phenotypes) for large strain libraries under fully controlled growth conditions. This was achieved by optimizing and automating the whole high-throughput fluxomics process and integrating all relevant software tools. This workflow allowed us to obtain highly detailed maps of carbon fluxes in the central carbon metabolism in a fully automated manner. It was applied to investigate the glucose fluxotypes of 180 Escherichia coli strains deleted for y-genes. Since the products of these y-genes potentially play a role in a variety of metabolic processes, the experiments were designed to be agnostic as to their potential metabolic impact. The obtained data highlight the robustness of E. coli's central metabolism to y-gene deletion. For two y-genes, deletion resulted in significant changes in carbon and energy fluxes, demonstrating the involvement of the corresponding y-gene products in metabolic function or regulation. This work also introduces novel metrics to measure the actual scope and quality of high-throughput fluxomics investigations.
- Subjects
GLUCOSE; METABOLIC regulation; CARBON metabolism; SOFTWARE development tools; FLUX (Energy)
- Publication
Metabolites (2218-1989), 2021, Vol 11, Issue 5, p271
- ISSN
2218-1989
- Publication type
Article
- DOI
10.3390/metabo11050271