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- Title
The DOF transcription factor Dof5.1 influences leaf axial patterning by promoting Revoluta transcription in Arabidopsis Hyung-Sae Kim et al. DOF regulates Revoluta transcription.
- Authors
Hyung-Sae Kim; Sung Jin Kim; Abbasi, Nazia; Bressan, Ray A.; Dae-Jin Yun; Sang-Dong Yoo; Suk-Yun Kwon; Sang-Bong Choi
- Abstract
Dof proteins are transcription factors that have a conserved single zinc finger DNA-binding domain. In this study, we isolated an activation tagging mutant Dof5.1-D exhibiting an upward-curling leaf phenotype due to enhanced expression of the REV gene that is required for establishing adaxial-abaxial polarity. Dof5.1-D plants also had reduced transcript levels for IAA6 and IAA19 genes, indicating an altered auxin biosynthesis in Dof5.1-D. An electrophoretic mobility shift assay using the Dof5.1 DNA-binding motif and the REV promoter region indicated that the DNA-binding domain of Dof5.1 binds to a TAAAGT motif located in the 5′-distal promoter region of the REV promoter. Further, transient and chromatin immunoprecipitation assays verified binding activity of the Dof5.1 DNA-binding motif with the REV promoter. Consistent with binding assays, constitutive over-expression of the Dof5.1 DNA-binding domain in wild-type plants caused a downward-curling phenotype, whereas crossing Dof5.1-D to a rev mutant reverted the upward-curling phenotype of the Dof5.1-D mutant leaf to the wild-type. These results suggest that the Dof5.1 protein directly binds to the REV promoter and thereby regulates adaxial-abaxial polarity.
- Subjects
PLANT genetics; TRANSCRIPTION factors; ARABIDOPSIS; GENETICS; PLANT proteins
- Publication
Plant Journal, 2010, Vol 64, Issue 3, p524
- ISSN
0960-7412
- Publication type
Article
- DOI
10.1111/j.1365-313X.2010.04346.x