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- Title
Ipomoea hederacea: an Imperative Source for Natural Antioxidants.
- Authors
Aziz-ur-Rehman; Abbas, Ansur; Riaz, Tauheeda; Ahmad, Shargeel; Shahzaman; Abbasi, Muhammad Athar; Siddiqui, Sabahat Zahra; Ajaib, Muhammad
- Abstract
Objective: The sole purpose of this study was to investigate the in-vitro antioxidant and radical scavenging potential of different organic fractions and aqueous fraction of ipomoea hederacea Jacq. Methods: Antioxidant constituents were initially extracted in methanol and then the methanolic extract was dissolved in distilled water and different fractions were sequentially prepared with the solvents like n-hexane, chloroform, ethyl acetate and n-butanol. Four methods were employed to measure the antioxidant power of plant fractions viz: 1,1-Diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity, ferric reducing antioxidant power (FRAP) assay, total antioxidant activity and Folin-Ciocalteau reagent assay for the determination of total phenolics. Results: From the results thus obtained, it was known that ethyl acetate soluble fraction showed the highest value of % inhibition of DPPH (83.26 % ± 0.60) at concentration of 125 μg/ml. The IC50 of this fraction was 60.28 ± 0.61 μg/ml, relative to the butylated 50 hydroxytoluene (BHT), having IC50 of 12.10 ± 1.29 μg/ml. It also displayed the highest 50 FRAP value (80 ± 0.3 μg TE/mL) the higher total antioxidant activity (0.25 ± 0.31) as well as highest total phenolic contents (62.35 ± 0.30 μg GAE/g) as compared to the other fractions. Conclusion: Ethyl acetate soluble fraction of Ipomoea hederacea Jacq. contains very good antioxidant compounds.
- Subjects
IPOMOEA; ANTIOXIDANTS; MEDICINAL plants; FREE radical scavengers; METHANOL
- Publication
Asian Journal of Pharmaceutical & Biological Research (AJPBR), 2011, Vol 1, Issue 4, p534
- ISSN
2231-2218
- Publication type
Article