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- Title
Zinc deficiency during in vitro maturation of porcine oocytes causes meiotic block and developmental failure.
- Authors
YUBYEOL JEON; JUNCHUL DAVID YOON; LIAN CAI; SEON-UNG HWANG; EUNHYE KIM; ZHONG ZHENG; EUIBAE JEUNG; EUNSONG LEE; SANG-HWAN HYUN
- Abstract
The present study investigated the effects of zinc deficiency during in vitro maturation (IVM) of porcine oocytes. Zinc deficiency was induced by administering the membrane-permeable zinc chelator N,N,N',N'-tetrakis-(2-pyridylmethyl)-ethylendiamine (TPEN). First, the effects of zinc deficiency during IVM on a TPEN-treated group and a TPEN+zinc-treated group compared with a control group were assessed. The oocyte maturation rates and subsequent embryonic developmental competence of the TPEN+zinc-treated oocytes were similar to those of the control oocytes (metaphase II [MII] rate, 93.0 and 92.7%, respectively, and blastocyst [BL] formation rate, 42.0 and 40.0%, respectively). These results were significantly different from those obtained for the TPEN-treated oocytes (MII rate, 0.61%; BL formation rate, 0%). Although the TPEN-treated oocytes were arrested at metaphase I (MI), the distribution of microtubules was normal. However, microfilament formation was abnormal in the TPEN-treated oocytes. Furthermore, the effect of a temporary zinc deficiency during IVM on oocyte maturation and subsequent embryonic development was assessed. TPEN (10 µM) was added to the IVM medium for 0, 7, 15 or 22 h. The 0 h-treated oocytes showed an 83.9% MII rate, while the 7 h-treated oocytes had a significantly lower MII rate (44.8%). Most of the 15- and 22 h-treated oocytes were arrested at MI (MI rate: 98.0 and 97.2%, respectively; MII rate, 0% in both groups). Reductions in the BL formation were dependent on the TPEN treatment duration (29.3, 9.2, 0, and 0% after 0, 7, 15 and 22 h, respectively). In conclusion, zinc is an essential element for successful oocyte maturation and embryonic development in pigs. Zinc deficiency caused a meiotic block and had lasting effects on early embryonic development.
- Subjects
SWINE embryology; ZINC deficiency diseases; DEVELOPMENTAL biology; OVUM; MEIOSIS; MICROTUBULES
- Publication
Molecular Medicine Reports, 2015, Vol 12, Issue 4, p5973
- ISSN
1791-2997
- Publication type
Article
- DOI
10.3892/mmr.2015.4125