We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
The Effect of Xylan Removal on the High-Solid Enzymatic Hydrolysis of Sugarcane Bagasse.
- Authors
Quintero, Leidy Patricia; de Souza, Nathalia P. Q.; Milagres, Adriane M. F.
- Abstract
Although high-solid enzymatic hydrolysis has emerged as an efficient strategy of sugar production from lignocellulosic materials, this process requires several steps to increase its efficiency. Current strategies include treatments to remove noncellulosic macromolecules. Here, dilute alkaline extraction was controlled to remove loosely bound xylan from sugarcane bagasse, and 14.7% (w/w) was recovered by ethanol precipitation. To the supernatant, sodium sulfite (5% w/w) was added to pretreat the residual sugarcane bagasse with alkaline sulfite ethanol (ASE). The sequential process allowed the removal of 85% of lignin, producing a carbohydrate-enriched solid. Incubation of ASE-pretreated bagasse with xylanase released up to 43% of the xylan as oligomers. Subsequent enzymatic saccharification was performed at initial solid loadings, 5, 10, and 15%. The conversion of ASE-pretreated bagasse yielded 84.4% glucan hydrolysis or 75 g/L glucose at 15% solids after 48 h, while samples with reduced xylan contents had lower water retention, and achieved relevant glucan hydrolysis yield (73%) and glucose titer (87 g/L). Enzymatic saccharification at high solid loading was dependent on xylan removal, and control of excess residual xylan may play an important role in fine-tuning product formation in high-solid enzymatic hydrolysis of the glucan fraction.
- Subjects
XYLANS; BAGASSE; GLUCANS; SUGARCANE; HYDROLYSIS; XYLANASES; MACROMOLECULES
- Publication
BioEnergy Research, 2022, Vol 15, Issue 2, p1096
- ISSN
1939-1234
- Publication type
Article
- DOI
10.1007/s12155-021-10294-0