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- Title
Effect of Metabolites of γ-Aminobutyric Shunt on Activities of NAD- and NADP-Isocitrate Dehydrogenases and Aconitate Hydratase from Higher Plants.
- Authors
Pinheiro de Carvalho, M. A. A.; Popova, T. N.; Dos Santos, T. M. M.; Zherebtsov, N. A.
- Abstract
We studied the influence of metabolites of γ-aminobutyric shunt of the tricarboxylic acid cycle on the activities of aconitate hydratase (EC.4.2.1.3) as well as NAD- and NADP-specific isocitrate dehydrogenases (EC.1.1.1.41 and EC.1.1.1.42, respectively) using purified enzyme preparations from pea leaves ( Pisum sativum L.) and maize scutellum ( Zea mays L.). γ-Aminobutyric acid and succinate proved to have no significant effect on these enzymes, while 0.1–0.2 mM glutamate markedly activated NADP isocitrate dehydrogenase from both sources, especially at unsaturating concentrations of the substrate. Succinic semialdehyde stimulated the activities of aconitate hydratase and NADP isocitrate dehydrogenase. The obtained data point to a similar pattern of the effect of intermediates of γ-aminobutyric shunt on the studied enzymatic activities for both photosynthetic tissues (pea leaves) and those with acidifying, transport, and digestive functions (maize scutellum). However, the absence of pronounced control effects of most metabolites on the studied enzymes allows us to assign them to a relatively inert pool of metabolites.
- Subjects
METABOLITES; AMINOBUTYRIC acid; DEHYDROGENASES; BIOMOLECULES; GLUTAMATE dehydrogenase; ACONITATE hydratase
- Publication
Biology Bulletin, 2003, Vol 30, Issue 3, p236
- ISSN
1062-3590
- Publication type
Article
- DOI
10.1023/A:1023899527163