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- Title
Coordinated control of a designed trans-acting ligase ribozyme by a loop–receptor interaction
- Authors
Matsumura, Shigeyoshi; Ohmori, Rei; Saito, Hirohide; Ikawa, Yoshiya; Inoue, Tan
- Abstract
Abstract: We previously developed a synthetic cis-acting RNA ligase ribozyme with 3′–5′ joining activity termed “DSL” (designed and selected ligase). DSL was easily transformed into a trans-acting form because of its highly modular architecture. In this study, we investigated the modular properties and turnover capabilities of a trans-acting DSL, tDSL-1/GUAA. tDSL-1/GUAA exhibited remarkably high activity compared with the parental cis-acting DSL, and it attained a high turnover number. Taken together, the results indicate that a loop–receptor interaction plays a significant role in determining the activity of the trans-acting ribozyme and in its ability to perform multiple turnovers of the reaction.
- Subjects
CATALYTIC RNA; LIGASES; CELL communication; CHEMICAL reactions; GEL electrophoresis; CELL receptors; GENETIC engineering
- Publication
FEBS Letters, 2009, Vol 583, Issue 17, p2819
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2009.07.036