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- Title
Poly(dG)–poly(dC) DNA appears shorter than poly(dA)–poly(dT) and possibly adopts an A-related conformation on a mica surface under ambient conditions
- Authors
Borovok, Natalia; Molotsky, Tatiana; Ghabboun, Jamal; Cohen, Hezy; Porath, Danny; Kotlyar, Alexander
- Abstract
Abstract: Three types of DNA: ∼2700bp polydeoxyguanylic olydeoxycytidylic acid [poly(dG)–poly(dC)], ∼2700bp polydeoxyadenylic polydeoxythymidylic acid [poly(dA)–poly(dT)] and 2686bp linear plasmid pUC19 were deposited on a mica surface and imaged by atomic force microscopy. Contour length measurements show that the average length of poly(dG)–poly(dC) is ∼30% shorter than that of poly(dA)–poly(dT) and the plasmid. This led us to suggest that individual poly(dG)–poly(dC) molecules are immobilized on mica under ambient conditions in a form which is likely related to the A-form of DNA in contrast to poly(dA)–poly(dT) and random sequence DNA which are immobilized in a form that is related to the DNA B-form.
- Subjects
ATOMIC force microscopy; NUCLEIC acids; GENES; THICKNESS measurement
- Publication
FEBS Letters, 2007, Vol 581, Issue 30, p5843
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2007.11.058