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- Title
Protein kinase D enzymes are dispensable for proliferation, survival and antigen receptor-regulated NFκB activity in vertebrate B-cells
- Authors
Liu, Ping; Scharenberg, Andrew M.; Cantrell, Doreen A.; Matthews, Sharon A.
- Abstract
Abstract: To investigate the importance of protein kinase D (PKD) enzymes we generated a PKD-null DT40 B-lymphocyte cell line. Previously we have shown that PKDs have an essential role in regulating class II histone deacetylases in DT40 B-cells [Matthews, S.A., Liu, P., Spitaler, M., Olson, E.N., McKinsey, T.A., Cantrell, D.A. and Scharenberg, A.M. (2006) Essential role for protein kinase D family kinases in the regulation of class II histone deacetylases in B lymphocytes. Mol. Cell Biol. 26, 1569–1577]. We now show that PKDs are also required to regulate HSP27 phosphorylation in DT40 B-cells. However, in contrast to previous observations in other cell types, PKD enzymes do not regulate basic cellular processes such as proliferation or survival responses, nor NFκB transcriptional activity downstream of the B cell antigen receptor. Thus, PKDs have a selective role in DT40 B-cell biology.
- Subjects
PROTEIN kinases; ENZYMES; PROTEINS; CATALYSTS
- Publication
FEBS Letters, 2007, Vol 581, Issue 7, p1377
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2007.02.055