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- Title
ALS2CL, the novel protein highly homologous to the carboxy-terminal half of ALS2, binds to Rab5 and modulates endosome dynamics
- Authors
Hadano, Shinji; Otomo, Asako; Suzuki-Utsunomiya, Kyoko; Kunita, Ryota; Yanagisawa, Yoshiko; Showguchi-Miyata, Junko; Mizumura, Hikaru; Ikeda, Joh-E
- Abstract
ALS2, the causative gene product for juvenile recessive amyotrophic lateral sclerosis (ALS2), is a guanine-nucleotide exchange factor for the small GTPase Rab5. Here, we report a novel ALS2 homologous gene, ALS2 C-terminal like (ALS2CL), which encodes a 108-kD ALS2CL protein. ALS2CL exhibited a specific but a relatively weak Rab5-GEF activity with accompanying rather strong Rab5-binding properties. In HeLa cells, co-expression of ALS2CL and Rab5A resulted in a unique tubulation phenotype of endosome compartments with significant colocalization of ALS2CL and Rab5A. These results suggest that ALS2CL is a novel factor modulating the Rab5-mediated endosome dynamics in the cells.
- Subjects
PROTEINS; NEURONS; MOTOR neuron diseases; G proteins
- Publication
FEBS Letters, 2004, Vol 575, Issue 1-3, p64
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2004.07.092