We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Lip b 1 is a novel allergenic protein isolated from the booklouse, Liposcelis bostrychophila.
- Authors
Ishibashi, O.; Sakuragi, K.; Fukutomi, Y.; Kawakami, Y.; Kamata, Y.; Sakurai, M.; Nakayama, S.; Uchiyama, H.; Kobayashi, H.; Kojima, H.; Inui, T.
- Abstract
Background Booklice, belonging to the order Psocoptera, are small household insect pests that are distributed worldwide. Liposcelis bostrychophila, a common home-inhabiting species of booklouse, infests old books, sheets of paper, and stored food. Recent entomological and serological studies demonstrated that L. bostrychophila accounted for the majority of detectable insects in house dust and could be a potent inducer of respiratory allergy. Our recent proteomic analysis identified a potent allergenic protein from L. bostrychophila, designated Lip b 1, and determined its partial amino acid sequences. Methods Cloning of c DNAs for Lip b 1 was performed by large-scale transcriptome analysis ( RNA-seq) and subsequent reverse transcription polymerase chain reaction. The full-length amino acid sequences deduced from Lip b 1 cDNAs were bioinformatically analyzed. The recombinant proteins of glutathione S-transferase ( GST)-fused Lip b 1 were analyzed by Western blot and enzyme-linked immunosorbent assay. Results Lip b 1 c DNAs encoding two types of 254-amino acid proteins were cloned. The clones shared 87% identity, and the deduced molecular weights and isoelectric points were consistent with those determined in our previous study. The two types of Lip b 1 proteins in the GST-fused form were similarly reactive with sera from allergic patients sensitized with L. bostrychophila. Conclusions Lip b 1 is a novel protein possibly causing booklouse allergy.
- Subjects
PSOCOPTERA; INSECT pest control; RESPIRATORY allergy; POLYMERASE chain reaction; RNA sequencing; GLUTATHIONE transferase
- Publication
Allergy, 2017, Vol 72, Issue 6, p918
- ISSN
0105-4538
- Publication type
Article
- DOI
10.1111/all.13091