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- Title
Specific analysis of the intron 22 XbaI polymorphism of the human factor VIII gene using long-distance PCR.
- Authors
De Brasi, Carlos D.; Bowen, Derrick J.; Collins, Peter W.; Larripa, Irene B.
- Abstract
A rapid, non-radioactive, PCR-based method to genotype the XbaI restriction fragment length polymorphism of the human factor VIII gene is described. The method uses long-distance PCR followed by XbaI restriction digestion and agarose gel electrophoresis. The 6.6 kb amplification product includes a constant XbaI site, which provides a digestion control. The specificity of the method was challenged by a blind experiment with 16 genomic DNA samples previously genotyped by Southern blot analysis: a perfect correlation was obtained between genotypes determined using Southern blot and PCR.
- Subjects
GENETIC polymorphisms; POLYMERASE chain reaction; GENES
- Publication
British Journal of Haematology, 1999, Vol 107, Issue 3, p566
- ISSN
0007-1048
- Publication type
Article
- DOI
10.1046/j.1365-2141.1999.01731.x