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- Title
THE SUSCEPTIBILITY OF RECENT ISOLATES OF Schistosoma mansoni TO PRAZIQUANTEL.
- Authors
MENDONÇA, Adriana Maria B.; FEITOSA, Ana Paula S.; VERAS, Dyana L.; MATOS-ROCHA, Thiago J.; dos Santos CAVALCANTI, Marília G.; BARBOSA, Constança Clara G. S.; BRAYNER, Fábio A.; ALVES, Luiz C.
- Abstract
Introduction: Schistosomiasis is a chronic disease caused by trematode flatworms of the genus Schistosoma and its control is dependent on a single drug, praziquantel (PZQ), but concerns over PZQ resistance have renewed interest in evaluating the in vitro susceptibility of recent isolates of Schistosoma mansoni to PZQ in comparison with well-established strains in the laboratory. Material and methods: The in vitro activity of PZQ (6.5-0.003 μg/mL) was evaluated in terms of mortality, reduced motor activity and ultrastructural alterations against S. mansoni. Results: After 3 h of incubation, PZQ, at 6.5 μg/mL, caused 100% mortality of all adult worms in the three types of recent isolates, while PZQ was inactive at concentrations of 0.08-0.003 μg/mL after 3 h of incubation. The results show that the SLM and Sotave isolates basically presented the same pattern of susceptibility, differing only in the concentration of 6.5 μg/mL, where deaths occurred from the range of 1.5 h in Sotave and just in the 3 h range of SLM. Additionally, this article presents ultrastructural evidence of rapid severe PZQ-induced surface membrane damage in S. mansoni after treatment with the drug, such as disintegration, sloughing, and erosion of the surface. Conclusion: According to these results, PZQ is very effective to induce tegument destruction of recent isolates of S. mansoni.
- Subjects
PRAZIQUANTEL; SCHISTOSOMA mansoni; ULTRASTRUCTURE of bacteria; MICROBIAL sensitivity tests; PLATYHELMINTHES; PARASITE behavior
- Publication
Revista do Instituto de Medicina Tropical de São Paulo, 2016, Vol 58, Issue 1, p1
- ISSN
0036-4665
- Publication type
Article
- DOI
10.1590/S1678-9946201658007