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- Title
Microirradiation techniques in radiobiological research.
- Authors
Drexler, Guido; Ruiz-Gómez, Miguel
- Abstract
The aim of this work is to review the uses of laser microirradiation and ion microbeam techniques within the scope of radiobiological research. Laser microirradiation techniques can be used for many different purposes. In a specific condition, through the use of pulsed lasers, cell lysis can be produced for subsequent separation of different analytes. Microsurgery allows for the identification and isolation of tissue sections, single cells and subcellular components, using different types of lasers. The generation of different types of DNA damage, via this type of microirradiation, allows for the investigation of DNA dynamics. Ion microbeams are important tools in radiobiological research. There are only a limited number of facilities worldwide where radiobiological experiments can be performed. In the beginning, research was mostly focused on the bystander effect. Nowadays, with more sophisticated molecular and cellular biological techniques, ion microirradiation is used to unravel molecular processes in the field of radiobiology. These include DNA repair protein kinetics or chromatin modifications at the site of DNA damage. With the increasing relevance of charged particles in tumour therapy and new concepts on how to generate them, ion microbeam facilities are able to address unresolved questions concerning particle tumour therapy.
- Subjects
RADIOBIOLOGY research; LASER beams; CHROMOSOME abnormalities; DNA damage; MICROSURGERY; DNA repair
- Publication
Journal of Biosciences, 2015, Vol 40, Issue 3, p629
- ISSN
0250-5991
- Publication type
Article
- DOI
10.1007/s12038-015-9535-3