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- Title
LncRNA-599554 sponges miR-15a-5p to contribute inductive ability of dermal papilla cells through positive regulation of the expression of Wnt3a in cashmere goat.
- Authors
Yin, Rong H.; Wang, Yan R.; Zhao, Su J.; Yin, Rong L.; Man Bai; Wang, Ze Y.; Zhu, Yu B.; Cong, Yu Y.; Liu, Hai Y.; Bai, Wen L.
- Abstract
Background: Long non-coding RNAs (lncRNAs), as post-transcriptional regulators, were thought to function in the inductive property of dermal papilla cells (DPCs) in cashmere goat. Previously, lncRNA-599554 was identified in secondary hair follicle (SHF) of cashmere goat, but its functional significance is unknown. Results: In the present investigation, we verified that lncRNA-599554 had significantly higher expression at the anagen dermal papilla of cashmere goat SHF than that at telogen. Based on overexpression and knockdown techniques, we found that lncRNA-599554 contributes the inductive property of DPCs of cashmere goat, which was assessed by detecting the changes in the expression of several typical indictor genes in DPCs including ET- 1, SCF, Versican, ALP, Lef1 and Ptc-1. Based on RNA pull-down assay, we verified that lncRNA-599554 directly interacted with chi-miR-15a-5p. Also, we showed that lncRNA-599554 positively regulated the Wnt3a expression in DPCs but which did not appear to involve its modulating of promoter methylation. Based on the use of Dual-luciferase reporter assays, our data indicated that lncRNA-599554 regulated the Wnt3a expression through chi-miR-15a-5p-mediated post-transcriptional level. Conclusions:Weshowed that lncRNA-599554 contributes the inductive property of DPCs in cashmere goatwhich might be achieved through sponging chi-miR-15b-5p to promote the Wnt3a expression. The results from the present investigation provided a novel insight into the functional mechanism of lncRNA-599554 in the SHF regeneration of cashmere goat along with the formation and growth of cashmere fiber.
- Subjects
CASHMERE; GOATS; HAIR follicles; NON-coding RNA; CELLS
- Publication
Electronic Journal of Biotechnology, 2020, Vol 45, p19
- ISSN
0717-3458
- Publication type
Article
- DOI
10.1016/j.ejbt.2020.03.002