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- Title
Characterization of Equine Adipose Tissue-Derived Stromal Cells: Adipogenic and Osteogenic Capacity and Comparison with Bone Marrow-Derived Mesenchymal Stromal Cells.
- Authors
VIDAL, MARTIN A.; KILROY, GAIL E.; LOPEZ, MANDI J.; JOHNSON, JILL R.; MOORE, RUSTIN M.; GIMBLE, JEFFREY M.
- Abstract
Objective To characterize equine adipose tissue-derived stromal cell (ASC) frequency and growth characteristics and assess of their adipogenic and osteogenic differentiation potential. Study Design In vitro experimental study. Animals Horses (n=5; aged, 9 months to 5 years). Methods Cell doubling characteristics of ASCs harvested from supragluteal subcutaneous adipose tissue were evaluated over 10 passages. Primary, second (P2), and fourth (P4) passage ASCs were induced under appropriate conditions to undergo adipogenesis and osteogenesis. Limit dilution assays were performed on each passage to determine the frequency of colony-forming units with a fibroblastic (CFU-F) phenotype and the frequency of ASC differentiation into the adipocyte (CFU-Ad) and osteoblast (CFU-Ob) phenotype. Results ASC isolates exhibited an average cell-doubling time of 2.1±0.9 days during the first 10 cell doublings. Approximately 1 in 2.3±0.4 of the total stromal vascular fraction nucleated cells were ASCs, based on the CFU-F assays, and 1 in 3.6±1.3 expressed alkaline phosphatase, an osteogenic marker. Primary ASCs differentiated in response to adipogenic (1 in 4.9±5.4, CFU-Ad) and osteogenic (1 in <2.44, CFU-Ob) inductive conditions and maintained their differentiation potential during subsequent passages (P2 and P4). Conclusion The frequency, in vitro growth rate, and adipogenic and osteogenic differentiation potential of equine ASCs show some differences to those documented for ASCs in other mammalian species. Clinical Relevance Adipose tissue is a potential source of adult stem cells for tissue engineering applications in equine veterinary medicine.
- Publication
Veterinary Surgery, 2007, Vol 36, Issue 7, p613
- ISSN
0161-3499
- Publication type
Article
- DOI
10.1111/j.1532-950X.2007.00313.x