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- Title
Evaluation of Wraps Covering the Distal Aspect of Pelvic Limbs for Prevention of Bacterial Strike-Through in an Ex Vivo Canine Model.
- Authors
VINCE, KENT J.; LASCELLES, B. DUNCAN X.; MATHEWS, KYLE G.; ALTIER, CRAIG; ROE, SIMON C.
- Abstract
Objective To determine differences in bacterial strike-through for materials commonly used to cover the distal aspect of the pelvic limb during operative site preparation. Study Design Randomized block design; ex vivo model. Animals Canine cadaveric pelvic limbs (n=40). Methods Pelvic limbs (n=40) were randomly assigned to 4 treatment groups: Group 1=Vetrap™+sterile Coban™; Group 2=latex glove+Vetrap™+sterile Coban™; Group 3=latex glove+Vetrap™+sterile Coban™+sterile latex glove+sterile Coban™; and Group 4=latex glove+Vetrap™+sterile disposable drape+sterile Coban™. Limbs were contaminated with a standardized bacterial solution and routinely prepared using the assigned distal leg wrap. Bandages were fluid challenged with a saline (0.9% NaCl) solution-soaked laparotomy sponge for 30 seconds. The wrap surface was sampled for microbial culture before surgical preparation, immediately after, and 60 minutes after applying a sterile leg wrap. Results Bacterial growth occurred in all Group 1 cultures, 90% of Group 2 cultures, and none of the Group 3 and 4 cultures, 60 minutes after applying the sterile wrap. Conclusion A distal leg wrap of Vetrap™+sterile Coban™ is not effective in preventing bacterial strike-through. Clinical Relevance If similar results occur in the live animal, then a sterile impermeable barrier must be incorporated into the distal leg wrap to prevent bacterial strike-through.
- Publication
Veterinary Surgery, 2008, Vol 37, Issue 4, p406
- ISSN
0161-3499
- Publication type
Article
- DOI
10.1111/j.1532-950X.2008.00395.x