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- Title
Characterizing lipid constituents of B. moojeni snake venom: a comparative approach for chemical and biological investigations.
- Authors
Carvalho, Nathalia Santos; Nardini, Viviani; Veronezes, Raul Moyses; Maciel, Jéssica Burlamaque; Trabuco, Amanda Cristina; De Carvalho, Mirian Félix; Fontanari, Caroline; Sartim, Marco Aurélio; de Moraes, Luiz Alberto Beraldo; Faccioli, Lúcia Helena
- Abstract
Snake venoms are complex mixtures majorly composed of proteins with well-studied biological effects. However, the exploration of non-protein components, especially lipids, remains limited despite their potential for discovering bioactive molecules. This study compares three liquid–liquid lipid extraction methods for both chemical and biological analyses of Bothrops moojeni snake venom. The methods evaluated include the Bligh and Dyer method (methanol, chloroform, water), considered standard; the Acunha method, a modification of the Bligh and Dyer protocol; and the Matyash method (MTBE/methanol/water), featuring an organic phase less dense than the aqueous phase. Lipidomic analysis using liquid chromatography with high-resolution mass spectrometry (LC-HRMS) system revealed comparable values of lipid constituents' peak intensity across different extraction methods. Our results show that all methods effectively extracted a similar quantity of lipid species, yielding approximately 17–18 subclasses per method. However, the Matyash and Acunha methods exhibited notably higher proportions of biologically active lipids compared to the Bligh and Dyer method, particularly in extracting lipid species crucial for cellular structure and function, such as sphingomyelins and phosphatidylinositol-phosphate. In conclusion, when selecting a lipid extraction method, it is essential to consider the study's objectives. For a biological approach, it is crucial to evaluate not only the total quantity of extracted lipids but also their quality and biological activity. The Matyash and Acunha methods show promise in this regard, potentially offering a superior option for extracting biologically active lipids compared to the Bligh and Dyer method.
- Publication
Archives of Toxicology, 2024, Vol 98, Issue 10, p3491
- ISSN
0340-5761
- Publication type
Article
- DOI
10.1007/s00204-024-03809-z