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- Title
Study of detection method and genetic characterization of tospoviruses.
- Authors
Okuda, Mitsuru
- Abstract
This article presents a study on the detection method and genetic characterization of tospoviruses. Tospoviruses within the family Bunyaviridae have quasi-spherical virions with lipid envelopes. Tomato spotted wilt virus is the type member of the genus Tospovirus. Fourteen distinct species are currently in this genus on the basis of their nucleotide sequence identities. Economic losses due to tospoviruses are quite high all over the world. In this study, an reverse transcription polymerase chain reaction approach using degenerated primers was developed to detect several tospovirus species, and genomic reassortants were analyzed to unveil the genetic factors responsible for symptoms of Watermelon silver mottle virus.
- Subjects
PLANT viruses; VIRUS diseases of plants; BUNYAVIRUSES; POLYMERASE chain reaction; REVERSE transcriptase; TOMATO spotted wilt virus disease
- Publication
Journal of General Plant Pathology, 2005, Vol 71, Issue 6, p458
- ISSN
1345-2630
- Publication type
Article
- DOI
10.1007/s10327-005-0225-0