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- Title
UV Light-Activated GdYVO<sub>4</sub>:Eu<sup>3+</sup> Nanoparticles Induce Reactive Oxygen Species Generation in Leukocytes Without Affecting Erythrocytes In Vitro.
- Authors
Onishchenko, Anatolii; Myasoedov, Valeriy; Yefimova, Svetlana; Nakonechna, Oksana; Prokopyuk, Volodymyr; Butov, Dmytro; Kökbaş, Umut; Klochkov, Vladimir; Maksimchuk, Pavel; Kavok, Nataliya; Tkachenko, Anton
- Abstract
Nanoparticles (NPs) have been reported to be promising enhancement agents for radiation therapy. The aim of the study was to assess the cytotoxicity of UV non-treated and UV pretreated GdYVO4:Eu3+ nanoparticles against erythrocytes and leukocytes by detecting eryptosis and reactive oxygen species (ROS) generation. Levels of intracellular ROS in erythrocytes and leukocytes using a ROS-sensitive dye 2ʹ,7ʹ-dichlorodihydrofluorescein diacetate (H2DCFDA), as well as eryptosis rate utilizing annexin V staining, following direct exposure to UV-activated and nonactivated NPs were detected by flow cytometry. Blood cells were collected from 9 intact WAG rats. Neither the UV light-untreated GdYVO4:Eu3+ NPs nor the treated ones promoted eryptosis and ROS generation in erythrocytes. Low concentrations of UV light-untreated NPs did not induce oxidative stress in leukocytes, evidenced by unaffected intracellular ROS levels. UV light treatment grants prooxidant properties to NPs, confirmed by NP-induced ROS overproduction in leukocytes. High concentrations of both UV light-treated and untreated NPs altered the redox state of leukocytes. UV light treatment imparts prooxidant properties to GdYVO4:Eu3+ NPs, making them promising radiosensitizing agents in cancer radiation therapy.
- Subjects
ERYTHROCYTES; REACTIVE oxygen species; LEUCOCYTES; BLOOD cells; NANOPARTICLES; ANNEXINS
- Publication
Biological Trace Element Research, 2022, Vol 200, Issue 6, p2777
- ISSN
0163-4984
- Publication type
Article
- DOI
10.1007/s12011-021-02867-z