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- Title
Deficient of LRRC8A attenuates hypoxia-induced necrosis in 3T3-L1 cells.
- Authors
Luo, Funong; Long, Keren; Li, Xiaokai; Mai, Miaomiao; Zhong, Zhining; Li, Side; Li, Penghao; Zhou, Shuling; Zhang, Tinghuan; Long, Xi; Jin, Long; Li, Mingzhou
- Abstract
Under acute hypoxia, multiple ion channels on the cell membrane are activated, causing cell swelling and eventually necrosis. LRRC8A is an indispensable protein of the volume-regulated anion channel (VRAC), which participates in swelling and the acceleration of cell necrosis. In this study, we revealed a dynamic change in the expression level of the LRRC8 family during hypoxia in 3T3-L1 cells. The disruption of LRRC8A in 3T3-L1 cells was also associated with a significant anti-necrotic phenotype upon hypoxia accompanied by the reduced expression of necrosis-related genes. In vivo, differential expression of LRRC8 family members was also identified between high-altitude pigs and their low-altitude relatives. Taken these findings together, this study demonstrates the involvement of LRRC8A in hypoxia-induced cell necrosis. Deletion of LRRC8A makes cells resistant to hypoxia-induced necrosis
- Subjects
CELLS; CELL membranes
- Publication
Bioscience, Biotechnology & Biochemistry, 2020, Vol 84, Issue 6, p1139
- ISSN
0916-8451
- Publication type
Article
- DOI
10.1080/09168451.2020.1730689