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- Title
Visualization and In Situ Ablation of Intracellular Bacterial Pathogens through Metabolic Labeling.
- Authors
Hu, Fang; Qi, Guobin; Kenry; Mao, Duo; Zhou, Shiwei; Wu, Min; Wu, Wenbo; Liu, Bin
- Abstract
Protected by the host cells, the hidden intracellular bacteria are typically difficult to kill by common antibiotics and cannot be visualized without complex cellular pretreatments. Herein, we successfully developed a bacteria‐metabolizable dual‐functional probe TPEPy‐d‐Ala, which is based on d‐alanine and a photosensitizer with aggregation‐induced emission for fluorescence turn‐on imaging of intracellular bacteria in living host cells and photodynamic ablation in situ. Once metabolically incorporated into bacterial peptidoglycan, the intramolecular motions of TPEPy‐d‐Ala are inhibited, leading to an enhanced fluorescent signal, which allows the clear visualization of the intracellular bacteria. Moreover, TPEPy‐d‐Ala can effectively ablate the labeled intracellular bacteria in situ owing to covalent ligation to peptidoglycan, yielding a low intracellular minimum inhibitory concentration (MIC) of 20±0.5 μg mL−1, much more efficient than that of a commonly used antibiotic, vancomycin.
- Subjects
INTRACELLULAR pathogens; BACTERIAL cell walls; VISUALIZATION; DRUG labeling; ANTIBIOTICS; LABELS
- Publication
Angewandte Chemie, 2020, Vol 132, Issue 24, p9374
- ISSN
0044-8249
- Publication type
Article
- DOI
10.1002/ange.201910187