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- Title
2,5-Diketo- D-gluconate production by a mixed culture of two newly-isolated strains: Flavimonas oryzihabitans and Pseudomonas cepacia.
- Authors
Sulo, Pavol; Hudecová, Daniela; Properová, Antónia; Bašnák, Ivan; Sedláček, Ivo
- Abstract
A mixed culture of two Gram-negative bacteria isolated from soil converted 50 g d-glucose l-1 to 2,5-diketo-d-gluconate (2,5 DKG) in 92% yield within 150 h. The first strain, producing 2-keto-d-gluconate (2 KDG) from d-glucose via d-gluconate (DG), was classified as Flavimonas oryzihabitans. The second strain, that converts 2 KDG to 2,5 DKG, was identified as Pseudomonas cepacia. This approach presents a new possibility to produce ascorbic acid by microbial transformation, including the use of other, more convenient substrates.
- Subjects
GRAM-negative bacteria; PSEUDOMONAS; CULTURES (Biology); GLUCOSE; VITAMIN C; MICROBIOLOGICAL synthesis
- Publication
Biotechnology Letters, 2001, Vol 23, Issue 9, p693
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1023/A:1010360603287