We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Limited protection of pneumococcal vaccines against emergent Streptococcus pneumoniae serotype 14/ST876 strains.
- Authors
Lan, Yinle; Liu, Lin; Hu, Dongping; Ge, Lihong; Xiang, Xi; Peng, Minfei; Fu, Ying; Wang, Yanfei; Li, Shuxian; Chen, Yan; Jiang, Yan; Tu, Yuexing; Vidal, Jorge E.; Yu, Yunsong; Chen, Zhimin; Wu, Xueqing
- Abstract
Purpose: Streptococcus pneumoniae (Spn) is a major cause of child death. We investigated the epidemiology of S. pneumoniae in a pediatric fever clinic and explored the genomics basis of the limited vaccine response of serotype 14 strains worldwide. Methods: Febrile disease and pneumonia were diagnosed following criteria from the WHO at the end of 2019 at a tertiary children's hospital. Spn was isolated by culture from nasopharyngeal (NP) swabs. The density was determined by lytA-base qPCR. Isolates were serotyped by Quellung and underwent antimicrobial susceptibility testing. Whole-genome sequencing was employed for molecular serotyping, MLST, antibiotic gene determination, SNP calling, recombination prediction, and phylogenetic analysis. Results: The presence of pneumococcus in the nasopharynx (87.5%, 7/8, p = 0.0227) and a high carriage (100%, 7/7, p = 0.0123) were significantly associated with pneumonia development. Living with siblings (73.7%, 14/19, p = 0.0125) and non-vaccination (56.0%, 28/50, p = 0.0377) contributed significantly to the Spn carriage. Serotype 14 was the most prevalent strain (16.67%, 5/30). The genome analysis of 1497 serotype 14 strains indicated S14/ST876 strains were only prevalent in China, presented limited vaccine responses with higher recombination activities within its cps locus, and unique variation patterns in the genes wzg and lrp. Conclusion: With the lifting of the one-child policy, it will be crucial for families with multiple children to get PCV vaccinations in China. Due to the highly variant cps locus and distinctive variation patterns in capsule shedding and binding proteins genes, the prevalent S14/ST876 strains have shown poor response to current vaccines. It is necessary to continue monitoring the molecular epidemiology of this vaccine escape clone.
- Subjects
CHINA; NASOPHARYNX microbiology; CARRIER state (Communicable diseases); GENOMICS; MICROBIAL sensitivity tests; PHYLOGENY; DATA analysis; RESPIRATORY infections; RESEARCH funding; SEROTYPES; VACCINE effectiveness; POLYMERASE chain reaction; TERTIARY care; CHILDREN'S hospitals; CHEST X rays; STREPTOCOCCUS; CHI-squared test; FEVER; LONGITUDINAL method; PNEUMOCOCCAL vaccines; ONE-way analysis of variance; STATISTICS; STREPTOCOCCAL diseases; COLLECTION &; preservation of biological specimens; DATA analysis software; HOSPITAL care of children; SEQUENCE analysis
- Publication
Infection, 2024, Vol 52, Issue 3, p801
- ISSN
0300-8126
- Publication type
Article
- DOI
10.1007/s15010-023-02110-y