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- Title
Maitake beta-glucan promotes recovery of leukocytes and myeloid cell function in peripheral blood from paclitaxel hematotoxicity.
- Authors
Hong Lin; de Stanchina, Elisa; Xi Kathy Zhou; Feng Hong; Seidman, Andrew; Fornier, Monica; Wei-Lie Xiao; Kennelly, Edward J.; Wesa, Kathleen; Cassileth, Barrie R.; Cunningham-Rundles, Susanna
- Abstract
Bone marrow myelotoxicity is a major limitation of chemotherapy. While granulocyte colony stimulating factor (G-CSF) treatment is effective, alternative approaches to support hematopoietic recovery are sought. We previously found that a beta-glucan extract from maitake mushroom Grifola frondosa (MBG) enhanced colony forming unit-granulocyte monocyte (CFU-GM) activity of mouse bone marrow and human hematopoietic progenitor cells (HPC), stimulated G-CSF production and spared HPC from doxorubicin toxicity in vitro. This investigation assessed the effects of MBG on leukocyte recovery and granulocyte/monocyte function in vivo after dose intensive paclitaxel (Ptx) in a normal mouse. After a cumulative dose of Ptx (90–120 mg/kg) given to B6D2F1mice, daily oral MBG (4 or 6 mg/kg), intravenous G-CSF (80 µg/kg) or Ptx alone were compared for effects on the dynamics of leukocyte recovery in blood, CFU-GM activity in bone marrow and spleen, and granulocyte/monocyte production of reactive oxygen species (ROS). Leukocyte counts declined less in Ptx + MBG mice compared to Ptx-alone ( p = 0.024) or Ptx + G-CSF treatment ( p = 0.031). Lymphocyte levels were higher after Ptx + MBG but not Ptx + G-CSF treatment compared to Ptx alone ( p < 0.01). MBG increased CFU-GM activity in bone marrow and spleen ( p < 0.001, p = 0.002) 2 days after Ptx. After two additional days (Ptx post-day 4), MBG restored granulocyte/monocyte ROS response to normal levels compared to Ptx-alone and increased ROS response compared to Ptx-alone or Ptx + G-CSF ( p < 0.01, both). The studies indicate that oral MBG promoted maturation of HPC to become functionally active myeloid cells and enhanced peripheral blood leukocyte recovery after chemotoxic bone marrow injury.
- Subjects
MAITAKE; BONE marrow; PLANT extracts; LEUCOCYTES; CANCER research
- Publication
Cancer Immunology, Immunotherapy, 2010, Vol 59, Issue 6, p885
- ISSN
0340-7004
- Publication type
Article
- DOI
10.1007/s00262-009-0815-3