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- Title
KfoE encodes a fructosyltransferase involved in capsular polysaccharide biosynthesis in Escherichia coli K4.
- Authors
Liu, Jia; Yang, Aihua; Liu, Jie; Ding, Xiaofan; Liu, Liming; Shi, Zhongping
- Abstract
Escherichia coli K4 synthesizes a capsular polysaccharide (CPS) consisting of a fructose-branched chondroitin (GalNAc-GlcA(fructose)n), which is a biosynthetic precursor of chondroitin sulfate. Here, the role of kfoE in the modification of the chondroitin backbone was investigated using knock-out and recombinant complementation experiments. kfoE disruption and complementation had no significant effect on cell growth. CPS production was increased by 15 % in the knock-out strain, and decreased by 21 % in the knock-out strain complemented with recombinant kfoE. CPS extracted from the knock-out strain was chondroitin, whereas CPS extracted from the complemented strain was a fructose-branched chondroitin. The results demonstrated that the kfoE gene product altered the fructose group at the C3 position of the GlcA residue during production of K4CPS.
- Subjects
ESCHERICHIA coli; FRUCTOSYLTRANSFERASES; POLYSACCHARIDE synthesis; FRUCTOSE; CHONDROITIN
- Publication
Biotechnology Letters, 2014, Vol 36, Issue 7, p1469
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1007/s10529-014-1502-9