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- Title
Enhanced intracellular Ca concentrations in Escherichia coli and Bacillus subtilis after addition of oligosaccharide elicitors.
- Authors
Murphy, Tania m.; Nilsson, Alex Y.; Roy, Ipsita; Harrop, Anthony; Dixon, Keith; Keshavarz, Tajalli
- Abstract
Elicitation can lead to overproduction of secondary metabolites in plants and microbes. Potential changes in cytosolic Ca levels in bacteria were studied in response to elicitation. We report, for the first time, the effect of oligosaccharide elicitors on intracellular Ca levels. The apoaequorin gene was cloned into Escherichia coli DH5α and Bacillus subtilis 1604 cultures. Addition of elicitors, oligoguluronate and mannan oligosaccharides, to the cultures caused up to 11-fold increase in cytosolic Ca in E. coli and tenfold increase in B. subtilis. These increases in Ca levels could therefore contribute to the enhancement of secondary metabolite levels.
- Subjects
INTRACELLULAR calcium; ESCHERICHIA coli; BACILLUS subtilis biotechnology; OLIGOSACCHARIDES; PLANT metabolites; CLONING
- Publication
Biotechnology Letters, 2011, Vol 33, Issue 5, p985
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1007/s10529-010-0511-6