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- Title
Rapid and low-cost diagnosis of Japanese yam mosaic virus infection in Chinese yam ( Dioscorea polystachya) leaves by a print-capture RT-PCR.
- Authors
Mochizuki, Tomofumi; Iwamoto, Itsuki; Atarashi, Aya; Ohki, Satoshi; Kikukawa, Hiroyuki
- Abstract
A rapid diagnostic method for Japanese yam mosaic virus (JYMV) infection in Chinese yam ( Dioscorea polystachya) by a print-capture RT-PCR was developed. The cut surface of a rolled leaf of a JYMV-infected Chinese yam was stamped onto a nitrocellulose membrane. The clipped membrane was boiled in eluting solution to elute the RNA for use as the template in RT-nested PCR. Although JYMV RNA was detectable in most infected plants in the first PCR of the print-capture RT-PCR, it was detected from all samples in the nested PCR. The tissue-printed membranes could be preserved for at least 3 months at 4 °C for JYMV detection.
- Subjects
DIAGNOSIS of plant diseases; MOSAIC viruses; DIOSCOREA batatas; REVERSE transcriptase polymerase chain reaction; NITROCELLULOSE
- Publication
Journal of General Plant Pathology, 2017, Vol 83, Issue 4, p189
- ISSN
1345-2630
- Publication type
Article
- DOI
10.1007/s10327-017-0719-6