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- Title
Development of a rapid and high-throughput molecular method for detecting the F200Y mutant genotype in benzimidazole-resistant isolates of Fusarium asiaticum.
- Authors
Duan, Yabing; Yang, Ying; Li, Tao; Zhao, Donglei; Cao, Junhong; Shi, Yiyuan; Wang, Jianxin; Zhou, Mingguo
- Abstract
Background The point mutation at codon 200 ( TTC→ TAC, F200Y) of the β2-tubulin gene confers resistance to benzimidazole fungicide in Fusarium asiaticum. These isolates with this mutation have been detected mainly by determining the minimum inhibitory concentration ( MIC) of fungicides, which is always time consuming, tedious and inefficient. Results A visual, rapid and efficient method with high specificity was developed, based on loop-mediated isothermal amplification ( LAMP). Six sets of LAMP primers were designed, and one set was optimised specifically to distinguish the F200Y mutant genotype. With the optimal LAMP primers, concentrations of LAMP components were optimised. The optimal reaction conditions were 57-64 °C for 75 min. The feasibility of the LAMP assay for detection of the F200Y mutant genotype of F. asiaticum was demonstrated by assaying diseased wheat spikelets that were artificially inoculated in the field. Conclusion The new LAMP assay had good specificity, sensitivity, stability and repeatability. It will be useful for assessing the risk of F. asiaticum populations with carbendazim resistance developing in the field, and will also provide important reference data for integrated control of Fusarium head blight caused by F. asiaticum. © 2016 Society of Chemical Industry
- Subjects
GENOTYPE-environment interaction; ECOLOGICAL genetics; FUSARIUM; PINE pitch canker; PEST control; PLANT physiology
- Publication
Pest Management Science, 2016, Vol 72, Issue 11, p2128
- ISSN
1526-498X
- Publication type
Article
- DOI
10.1002/ps.4243