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- Title
杀伤细胞对肺癌小鼠 Lewis 细胞增殖的抑制作用.
- Authors
姜伟华; 董跃华; 魏玉磊; 张月; 乔志飞; 张苏宁
- Abstract
Objective: To investigate inhibitory role of Lewis killer cells against the proliferation and growth of lung cancer cell. Methods: 46 male ICR mice aged 8 weeks, weighing 22-25 g were selected. 10 rats were randomly selected as normal control group, and the rest 30 rats were established the model, and when the model was successfully established, the rats were divided into two groups. The experimental group was intravenous injected of killer cells to 1× 107 /0.2 mL, once a day, and the rats of the model group and the control group received intravenous injection of saline. The level of TNF-α, IL-2 and IL-4 was detected by ELISA, and the apoptosis was detected by flow cytometry, and the inhibition rate of cancer cells was detected by MTT. Results: Compared with the control group, the right axillary tumor weight in experimental group was lower, and the difference was statistically significant (P<0.01). The Lewis human lung cancer cell presented a large amount of apoptosis in the experimental group, but almost no apoptosis was detectable in the model group with statistical significance between two groups (P<0.05). The spleen supernatant IL-2, TNF-α and IFN- gamma were significantly higher in the experimental group than that of the model group and normal control group, and the difference was statistically significant (P<0.01). Conclusion: Killer cells can inhibit the proliferation and growth of Lewis lung cancer cell through activating the lymphocytes and secreting the cytokines.
- Publication
Progress in Modern Biomedicine, 2015, Vol 15, Issue 25, p4856
- ISSN
1673-6273
- Publication type
Article
- DOI
10.13241/j.cnki.pmb.2015.25.013