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- Title
Static Aerated Composting of African Swine Fever Virus-Infected Swine Carcasses with Rice Hulls and Sawdust.
- Authors
Hutchinson, Mark; Duc, Hoang Minh; Flory, Gary A.; Ngan, Pham Hong; Son, Hoang Minh; Hoa, Tran Thi Khanh; Lan, Nguyen Thi; Rozeboom, Dale W.; Remmenga, Marta D.; Vuolo, Matthew; Miknis, Robert; Miller, Lori P.; Burns, Amira; Flory, Renée
- Abstract
Identifying and ensuring the inactivation of the African Swine Fever virus in deadstock is a gap in the swine industry's knowledge and response capabilities. The results of our study demonstrate that ASFv in deadstock was inactivated using static aerated composting as the carcass disposal method. Replicated compost piles with whole market hogs and two different carbon sources were constructed. In-situ bags containing ASFv-infected spleen tissue were placed alongside each of the carcasses and throughout the pile. The bags were extracted at days 0, 1, 3, 7, 14, 28, 56, and 144 for ASFv detection and isolation. Real-time PCR results showed that DNA of ASFv was detected in all samples tested on day 28. The virus concentration identified through virus isolation was found to be below the detection limit by day 3 in rice hulls and by day 7 in sawdust. Given the slope of the decay, near-zero concentration with 99.9% confidence occurred at 5.0 days in rice hulls and at 6.4 days in sawdust. Additionally, the result of virus isolation also showed that the virus in bone marrow samples collected at 28 days was inactivated.
- Subjects
RICE hulls; AFRICAN swine fever; SWINE carcasses; CLASSICAL swine fever; AFRICAN swine fever virus; WOOD waste
- Publication
Pathogens, 2023, Vol 12, Issue 5, p721
- ISSN
2076-0817
- Publication type
Article
- DOI
10.3390/pathogens12050721