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- Title
Detection and inhibition of IgE antibodies reactive with cross‐reactive carbohydrate determinants in an ELISA for allergen‐specific IgE in horses.
- Authors
Enck, Kevin M.; Lee, Kenneth W.; McKinney, Brennan H.; Blankenship, Karen D.; Montesano, Cathe
- Abstract
Background: It has been demonstrated that immunoglobulin (Ig)E specific for cross‐reactive carbohydrate determinants (CCD) is present in the serum of sensitized humans, dogs and cats, and that these CCD‐specific antibodies might confound serological testing. Hypothesis/Objective: The objective was to determine whether or not CCD‐reactive antibodies occur in horses and to investigate the prevalence of CCD‐reactive IgE antibodies in equine sera using a monoclonal cocktail‐based enzyme‐linked immunosorbent assay designed to detect allergen‐specific IgE in horses, and to evaluate a means for successful inhibition of these CCD. Methods and materials: Sera from 28 horses suspected of clinical allergy were evaluated, with and without a proprietary inhibitor which contains carbohydrates derived from bromelain (BROM‐CCD), using a panel of 72 allergens that include 15 grasses, 17 trees, nine weeds, five mites, 12 fungi, 12 insects and two environmental allergens. Results: Twenty‐five samples were shown to be reactive to at least one of the allergens, and 15 were reactive to 10 allergens or more. BROM‐CCD had minimal effect on the mite reactivity in any of the positive samples; however, substantial inhibition for pollen allergens (trees, grasses and weeds) was demonstrable. Reduction in signal to pollens ranged from 20% to 100% for samples that were inhibited by CCD‐BROM. Conclusions and Clinical Importance: These results demonstrate that CCD‐reactive IgE antibodies are evident in horses and that BROM‐CCD can be effective in reducing reactions with these irrelevant carbohydrates and will likely yield a more accurate in vitro allergen reactivity profile for selection of allergens included in an immunotherapeutic regime.
- Subjects
IMMUNOGLOBULIN E; MONOCLONAL antibodies; IMMUNOGLOBULINS; CARBOHYDRATES; ENZYME-linked immunosorbent assay; HORSES; SERODIAGNOSIS
- Publication
Veterinary Dermatology, 2021, Vol 32, Issue 6, p685
- ISSN
0959-4493
- Publication type
Article
- DOI
10.1111/vde.12963