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- Title
Dissection of σ<sup>E</sup>-dependent cell lysis in Escherichia coli: roles of RpoE regulators RseA, RseB and periplasmic folding catalyst PpiD.
- Authors
Noor, Rashed; Murata, Masayuki; Nagamitsu, Hiroshi; Klein, Gracjana; Raina, Satish; Yamada, Mamoru
- Abstract
To understand the mechanism of σE-dependent cell lysis, we examined the consequences of deletion derivatives of rpoE regulators rseA, rseB and rseC on σE transcription, on levels of free versus membrane-bound σE and on OMP-biogenesis limiting factor(s) that could impact cell lysis. RT-PCR showed that individual nonpolar Δ rseA and Δ rseB increased the rpoE expression to varying extents, with pronounced induction in Δ rseA. Significantly the ratio of soluble (free) versus membrane-bound form of RpoE increased in Δ rseA, however without increase of its total amount, unraveling furthermore complexity in RpoE regulation. Significant characteristics of cell lysis, accompanied by a severe reduction in the levels of periplasmic OMP-folding factor (PpiD), were observed in Δ rseA. The cell-lysis phenotype of Δ rseA was suppressed by either rseA or ppiD plasmids, but neither by rseB nor by rseC clones. However, the cell lysis of the wild-type strain was almost completely repressed not only by the rseA clone but also by the rseB clone, suggesting RseB might be limiting in vivo. Thus, increase in the ratio of free σE in rseA mutants with a concomitant reduction in PpiD levels can account for σE-dependent lysis in concert with a potential role of small RNAs on the lysis process.
- Subjects
ESCHERICHIA coli; GENOTYPE-environment interaction; PLASMIDS; GENETIC regulation; PROTEIN folding
- Publication
Genes to Cells, 2009, Vol 14, Issue 7, p885
- ISSN
1356-9597
- Publication type
Article
- DOI
10.1111/j.1365-2443.2009.01318.x