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- Title
A codominant SCAR marker linked to the genic male sterility gene ( ms<sub> 1</sub>) in chili pepper ( Capsicum annuum).
- Authors
Lee, J.; Yoon, J. B.; Han, J.‐H.; Lee, W. P.; Do, J. W.; Ryu, H.; Kim, S. H.; Park, H. G.
- Abstract
With 3 figures As one of the genic male sterility (GMS) materials in chili pepper ( Capsicum annuum L.), GMS1 has been used for commercial F1 hybrid seed production. The male sterility of GMS1 is controlled by a recessive nuclear gene, named ms 1. In this study, we developed DNA markers linked to the ms 1 locus using a combination of bulked segregant analysis and amplified fragment length polymorphism (AFLP) in a segregating sibling population. From the screening of 1024 AFLP primer combinations, the AFLP marker E-AGC/M-GTG (514 bp) was identified as being linked to the ms 1 locus at a distance of about 3 cM. Based on internal sequencing analysis of the E-AGC/M-GTG marker between male fertile and sterile plants, we identified three small deletions with a size of altogether 42 bp in the male-fertile plant and developed a codominant sequence characterized amplified region (SCAR) marker. This SCAR marker may be valuable for marker-assisted breeding in the hybrid seed production system of chili pepper using the GMS1 line.
- Subjects
MALE sterility in plants; STERILITY in plants; CAPSICUM annuum; PEPPERS; GENETIC markers
- Publication
Plant Breeding, 2010, Vol 129, Issue 1, p35
- ISSN
0179-9541
- Publication type
Article
- DOI
10.1111/j.1439-0523.2009.01643.x