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- Title
Magnetite/Rhodamine 6G nanoparticles internalization in Neurospora crassa cells: towards the magnetic hyperthermia application.
- Authors
Hernández-Guerrero, Nadia; Castro-Longoria, Ernestina; Torres-Gómez, Nayely; Ruiz, Víctor Fabián; Arenas-Alatorre, Jesús; Martínez-Mondragón, María Magdalena; Vilchis-Nestor, Alfredo Rafael
- Abstract
In this work, the study of aqueous media co-precipitation-obtained magnetite nanoparticles (Fe3O4-NPs) and Rhodamine 6G coating nanoparticles: Fe3O4/Rh6G-NPs, stabilized with polyethylene glycol is presented. To assess their suitability for magnetic hyperthermia application, cellular internalization of Fe3O4/Rh6G nanoparticles using the fungus Neurospora crassa as a model system was also explored. The structural characterization by Infrared and Raman spectroscopies, as well as Transmission Electron Microscopy (TEM) of this material showed that the Fe3O4/Rh6G-NPs are chemically stable with a pure crystalline phase, exhibiting an average particle size of 38.6 ± 6.4 nm and a near-spherical shape; all these properties are desired for nanoparticles that are intended to be used in biomedical applications. Additionally, a marked superparamagnetic behavior in the composite was revealed by the magnetic characterization. The living cells exposed to Fe3O4/Rh6G-NPs showed fluorescence from the apical dome to the basal zone of the hyphae scanned under confocal microscopy; while TEM observations of ultrathin sections demonstrated the presence of Fe3O4/Rh6G-NPs throughout the cytoplasm of the fungus, corroborating internalization of the nanocomposite. It is worth mentioning that alteration of fungus growth or cell morphology after exposure to Fe3O4/Rh6G-NPs were not observed, which represent an important precedent of internalization of inorganic materials-based nanocomposites in cells for further exploration of magnetic hyperthermia applications.
- Subjects
NEUROSPORA crassa; MAGNETICS; MAGNETITE; NANOPARTICLES; TRANSMISSION electron microscopy
- Publication
Applied Nanoscience, 2022, Vol 12, Issue 6, p1791
- ISSN
2190-5509
- Publication type
Article
- DOI
10.1007/s13204-021-02317-1