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- Title
CD30-mediated cell cycle arrest associated with induced expression of p21<sup>CIP1/WAF1</sup> in the anaplastic large cell lymphoma cell line Karpas 299.
- Authors
Hübinger, Gabriele; Müller, Elke; Scheffrahn, Inka; Schneider, Christof; Hildt, Eberhard; Singer, Bernhard B; Sigg, Irmgard; Graf, Jochen; Bergmann, Lothar
- Abstract
One of the major characteristics of anaplastic large cell lymphomas (ALCL) is the expression of the Ki-1/CD30 antigen. While the receptor mediates NF-κB-activation in Hodgkin's lymphomas, some data suggest the CD30-mediated apoptosis of other CD30-expressing cells. We were able to demonstrate that activation of CD30 leads to different effects regarding cell proliferation of the ALCL-derived cell lines Karpas 299 and JB6. Western and Northern blotting analysis revealed that CD30-induced growth inhibition of Karpas 299 cells correlated with a strong upregulation of the cell cycle inhibitor p21CIP1/WAF1. We found a non activating point mutation at codon 273 in exon 8 of the p53 gene in Karpas 299 cells which indicates an p53-independent mechanism for induced p21 expression. Abundant p21 protein expression resulted in hypophosphorylation of the retinoblastoma protein (Rb) and inhibition of the proliferating cell nuclear antigen (PCNA). CD30-stimulated cells showed no indications of apoptotic cell death, like genomic DNA fragmentation or cleavage of the caspase-3 target protein poly (ADP-ribose) polymerase (PARP). Our results indicate that CD30 is able to mediate an p21-associated cell cycle arrest in ALCL with possible implications for prognosis and clinical treatment. Oncogene (2001) 20, 590–598.
- Subjects
CELL cycle; LYMPHOMAS; APOPTOSIS; CELL death
- Publication
Oncogene, 2001, Vol 20, Issue 5, p590
- ISSN
0950-9232
- Publication type
Article