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- Title
The subiculum: a Potential site of ictogenesis in a neonatal seizure Model.
- Authors
Xin-Xin Wang; Yong-Hua Li; Hai-Qing Gong; Pei-Ji Liang; Pu-Ming Zhang; Qin-Chi Lu
- Abstract
Studies have reported that the subiculum is one origin of interictal-like discharges inadult patients with temporal lobe epilepsy; however, whether the subiculum representsa site of ictogenesis for neonatal seizures remains unclear. In this study, multi-electroderecording techniques were used to record epileptiform discharges induced by low-Mg2+or high-K+ artificial cerebrospinal fluid in neonatal mouse hippocampal slices, and thespatiotemporal dynamics of the epileptiform discharges were analyzed. The Na+-K+-2Cl-cotransporter 1 (NKCC1) blocker, bumetanide, was applied to test its effect upon epileptiformdischarges in low-Mg2+ model. The effect of N-methyl-d-aspartate receptors(NMDARs) antagonist, d-AP5, upon the epileptiform discharges in high-K+ model wasexamined. We found that the neonatal subiculum not only relayed epileptiform dischargesemanating from the hippocampus proper (HP) but also initiated epileptiformdischarges (interictal- and ictal-like discharges) independently. The latency to onset of the first epileptiform discharge initiated in the subiculum was similar to that initiated inthe HP. Bumetanide efficiently blocked seizures in the neonatal HP, but was less effectivelyin suppressing seizures initiated in the subiculum. In high-K+ model, d-AP5 wasmore effective in blocking seizures initiated in the subiculum than that initiated in the HP.Furthermore, Western blotting analysis showed that NKCC1 expression was lower inthe subiculum than that in the HP, whereas the expression of NMDAR subunits, NR2Aand NR2B, was higher in the subiculum than that in the HP. Our results revealed that thesubiculum was a potential site of ictogenesis in neonatal seizures and possessed similarseizure susceptibility to the HP. GABAergic excitation resulting from NKCC1 may play aless dominant role during ictogenesis in the subiculum than that in the HP. The subicularictogenesis may be related to the glutamatergic excitation mediated by NMDARs.
- Subjects
INFANTILE spasms; TEMPORAL lobe epilepsy; CEREBROSPINAL fluid; PATIENTS
- Publication
Frontiers in Neurology, 2017, Vol 8, p1
- ISSN
1664-2295
- Publication type
Case Study
- DOI
10.3389/fneur.2017.00147