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- Title
Capsule Typing of Haemophilus influenzae by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry.
- Authors
Månsson, Viktor; Gilsdorf, Janet R.; Kahlmeter, Gunnar; Kilian, Mogens; Kroll, J. Simon; Riesbeck, Kristian; Resman, Fredrik
- Abstract
Encapsulated Haemophilus influenzae strains belong to type-specific genetic lineages. Reliable capsule typing requires PCR, but a more efficient method would be useful. We evaluated capsule typing by using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. Isolates of all capsule types (a-f and nontypeable; n = 258) and isogenic capsule transformants (types a-d) were investigated. Principal component and biomarker analyses of mass spectra showed clustering, and mass peaks correlated with capsule type-specific genetic lineages. We used 31 selected isolates to construct a capsule typing database. Validation with the remaining isolates (n = 227) showed 100% sensitivity and 92.2% specificity for encapsulated strains (a-f; n = 61). Blinded validation of a supplemented database (n = 50) using clinical isolates (n = 126) showed 100% sensitivity and 100% specificity for encapsulated strains (b, e, and f; n = 28). MALDI-TOF mass spectrometry is an accurate method for capsule typing of H. influenzae.
- Subjects
HAEMOPHILUS influenzae; HAEMOPHILUS diseases; MATRIX-assisted laser desorption-ionization; LINEAGE; BIOMARKERS; BACTERIOPHAGE typing; COMPARATIVE studies; BIOLOGICAL evolution; GENETICS; MASS spectrometry; RESEARCH methodology; MEDICAL cooperation; POLYMERASE chain reaction; RESEARCH; RESEARCH evaluation; EVALUATION research; BACTERIAL capsules; SEQUENCE analysis
- Publication
Emerging Infectious Diseases, 2018, Vol 24, Issue 3, p443
- ISSN
1080-6040
- Publication type
journal article
- DOI
10.3201/eid2403.170459