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- Title
Effect Assessment of Aurantio-Obtusin on Novel Human Renal Glomerular Endothelial Cells Model Using a Microfluidic Chip.
- Authors
Qin, Wen; Yang, Zhuo; Yin, Jiyong; Chen, Di; Huo, Junsheng; Wang, Jingbo; Wang, Liyuan; Zhuo, Qin
- Abstract
Cassiae semen is widely used as a raw material of health food. Anthraquinone compounds, the main components in cassiae semen, have been reported to show nephrotoxicity. Aurantio-obtusin (AO) is a major anthraquinone compound extracted from cassiae semen. This study investigates the effects of AO on the morphology and physiological function of human renal glomerular endothelial cells (HRGECs) on a microfluidic chip device for the first time. HRGECs were cultured on a microfluidic plate and exposed to a series of AO concentrations. Compared with traditional 96-well culture, HRGECs cultured on the microfluidic chip appeared to better mimic the glomerular microenvironment in vivo. AO induced different degrees of damage to cellular morphology and physiological function. The leakage of lactate dehydrogenase (LDH), as well as the secretion of interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), transforming growth factor-β1 (TGF-β1), and monocyte chemoattractant protein 1 (MCP-1), increased in the AO treated groups. At the same time, cell viability and expression of ZO-1 in the AO treated groups decreased in a dose-dependent manner. The innovative device enables direct visualization and quantification to evaluate the cytotoxic effects of AO on HRGECs, and provides a useful visual in vitro model for studying health effect of health food.
- Subjects
QUINONE; ENDOTHELIAL cells; NEPHROTOXICOLOGY; IN vitro studies; INTERLEUKINS; TRANSFORMING growth factors-beta; CELL culture; MICROFLUIDIC analytical techniques; CELL survival; GENE expression; TOXICITY testing; LACTATE dehydrogenase; TUMOR necrosis factors; PLANT extracts; NATURAL foods; INFLAMMATORY mediators; KIDNEY glomerulus; PHARMACODYNAMICS
- Publication
Nutrients, 2022, Vol 14, Issue 21, p4615
- ISSN
2072-6643
- Publication type
Article
- DOI
10.3390/nu14214615