We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Protective Effects of Collagen Tripeptides in Human Aortic Endothelial Cells by Restoring ROS-Induced Transcriptional Repression.
- Authors
Saito-Takatsuji, Hidehito; Yoshitomi, Yasuo; Ishigaki, Yasuhito; Yamamoto, Shoko; Numata, Noriaki; Sakai, Yasuo; Takeuchi, Masayoshi; Tomosugi, Naohisa; Katsuda, Shogo; Yonekura, Hideto; Ikeda, Takayuki
- Abstract
Collagen tripeptide (CTP) is defined as a functional food material derived from collagenase digests of type I collagen and contains a high concentration of tripeptides with a Gly-X-Y sequence. CTP has several biological effects, including the acceleration of fracture healing, ameliorating osteoarthritis, and improving dryness and photoaging of the skin. Recently, an antiatherosclerotic effect of CTP has been reported, although its molecular mechanism is yet to be determined. In this study, we examined the effects of CTP on primary cultured human aortic endothelial cells (HAECs) under oxidative stress, because oxidative endothelial dysfunction is a trigger of atherosclerosis. DNA microarray and RT-qPCR analyses showed that CTP treatment recovered the downregulated expression of several genes, including the interleukin-3 receptor subunit alpha (IL3RA), which were suppressed by reactive oxygen species (ROS) treatment in HAECs. Furthermore, IL3RA knockdown significantly decreased the viability of HAECs compared with control cells. RT-qPCR analysis also showed that solute carrier 15 family peptide transporters, which are involved in CTP absorption into cells, were expressed in HAECs at levels more than comparable to those of a CTP-responsive human osteoblastic cell line. These results indicated that CTP exerts a protective effect for HAECs, at least in part, by regulating the recovery of ROS-induced transcriptional repression.
- Subjects
DNA analysis; ATHEROSCLEROSIS prevention; COLLAGEN; ENDOTHELIAL cells; FUNCTIONAL foods; REVERSE transcriptase polymerase chain reaction; INTERLEUKINS; MICROARRAY technology; GENE expression; OXIDATIVE stress; CELL survival; REACTIVE oxygen species; POLYMERASE chain reaction; PEPTIDES
- Publication
Nutrients, 2021, Vol 13, Issue 7, p2226
- ISSN
2072-6643
- Publication type
Article
- DOI
10.3390/nu13072226