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- Title
Effects of Different Doses of Fructooligosaccharides (FOS) on the Composition of Mice Fecal Microbiota, Especially the Bifidobacterium Composition.
- Authors
Mao, Bingyong; Gu, Jiayu; Li, Dongyao; Cui, Shumao; Zhao, Jianxin; Zhang, Hao; Chen, Wei
- Abstract
Fructooligosaccharides (FOS) are a well-known class of prebiotic and are considered to selectively stimulate the growth of bifidobacteria in the gut. Previous studies focused on the growth stimulation of <italic>Bifidobacterium</italic>, but they did not further investigate the bifidobacterial composition and the specific species that were stimulated. In this study, mice were fed with FOS in different doses for four weeks and the composition of fecal microbiota, in particular <italic>Bifidobacterium</italic>, was analyzed by sequencing the V3–V4 region and the <italic>groEL</italic> gene on the MiSeq platform, respectively. In the high-dose group, the relative abundance of Actinobacteria was significantly increased, which was mainly contributed by <italic>Bifidobacterium</italic>. At the genus level, the relative abundances of <italic>Blautia</italic> and <italic>Coprococcus</italic> were also significantly increased. Through the <italic>groEL</italic> sequencing, 14 species of <italic>Bifidobacterium</italic> were identified, among which <italic>B. pseudolongum</italic> was most abundant. After FOS treatment, <italic>B. pseudolongum</italic> became almost the sole bifidobacterial species (>95%). <italic>B. pseudolongum</italic> strains were isolated and demonstrated their ability to metabolize FOS by high performance liquid chromatography (HPLC). Therefore, we inferred that FOS significantly stimulated the growth of <italic>B. pseudolongum</italic> in mice. Further investigations are needed to reveal the mechanism of selectiveness between FOS and <italic>B. pseudolongum</italic>, which would aid our understanding of the basic principles between dietary carbohydrates and host health.
- Subjects
FECAL analysis; ANIMAL experimentation; BIFIDOBACTERIUM; FECES; CARBOHYDRATE content of food; FRUCTOSE; HIGH performance liquid chromatography; OLIGOSACCHARIDES; MICE; GUT microbiome; ACTINOBACTERIA; SEQUENCE analysis
- Publication
Nutrients, 2018, Vol 10, Issue 8, p1105
- ISSN
2072-6643
- Publication type
Article
- DOI
10.3390/nu10081105